J. Charlwood et al., 2-DIMENSIONAL CHROMATOGRAPHY IN THE ANALYSIS OF COMPLEX GLYCANS FROM TRANSFERRIN, Analytical chemistry (Washington), 70(13), 1998, pp. 2530-2535
Oligosaccharides released from human transferrin have been derivatized
with 2-aminoacridone (2-AMAC) prior to analysis by either reversed- o
r normal-phase highperformance liquid chromatography. These separation
methods are complementary and allow distinction between isomeric mono
- and disialylated oligosaccharides, which terminate with Sia alpha 2-
6Gal or Sia alpha 2-3Gal at the nonreducing end. Collected fractions o
f 2-AMAC-derivatized glycans were analyzed by matrix-assisted laser de
sorption/ionization time-of-flight mass spectrometry, before and after
desialylation.