INVESTIGATION OF ENANTIOMER BONDING ON A CHIRAL STATIONARY-PHASE BY FT-RAMAN SPECTROMETRY

The bonding of serine, phenylalanine, and mandelic acid enantiomers on an N-3,5-dinitrobenzoyl-L-leucine chiral stationary phase (on zeolite A support) has been investigated by FT-Raman spectrometry. It was fou nd that retention is due to hydrogen bonds and pi-stacking interaction s between the stationary phase and the analyte. The involvement of the two different amide groups (as donor and/or acceptor) in the complexa tion reaction can be followed based on spectral data. A correlation wa s found between the ratio of the amide I and the ring stretching (1532 cm(-1)) bands and retention data.