Rj. Stevens et al., SOIL-PH AFFECTS THE PROCESSES REDUCING NITRATE TO NITROUS-OXIDE AND DI-NITROGEN, Soil biology & biochemistry, 30(8-9), 1998, pp. 1119-1126
Nitrous oxide can be produced by nitrification, denitrification or dis
similatory NO3- reduction to NH4+ (DNRA), whereas N-2 can only be prod
uced by denitrification. Nitrite is a common intermediate in ail three
processes. high pH favouring NO2- accumulation and DNRA. During denit
rification the mole fraction of N2O decreases as pH increases, but lit
tle is known about the effect of soil pH on the production of N2O duri
ng DNRA, and on the relative importance of nitrification, denitrificat
ion and DNRA to N-gas fluxes. We investigated the processes responsibl
e for the production of N2O and N-2 in one soil at pH values of 5.6, 6
.0, 6.5 and 8.0. A range of aeration conditions were created by 24 h i
ncubations at 20 degrees C with factorial combination of two rates of
NO3- (1.0 and 4.4 mu mol N g(-1) oven-dry soil), two rates of C (20 an
d 80 mu mol C g(-1) oven-dry soil), and water to attain three soil moi
sture contents (50, 65, 80% WFPS). The added NO3- was labelled at 40 a
tom% excess N-15. Automated isotope-ratio mass spectrometry was used t
o determine the fluxes of N-2 and N2O. and the source of the N2O. The
flux of N-2 increased with pH but the effect of pH on the flux of N2O
was inconsistent. The maximum flux of N2O occurred at pH 6.5 and the m
inimum fluxes at pH 6.0 and 8.0. The dominant source of N-2 and N2O wa
s from the NO3- pool. There was evidence that DNRA was occurring as we
ll as denitrification particularly at the higher pH values. At pH 8 NO
2- accumulated and there was a direct relationship between N2O flux an
d NO2- concentration. The N-15 content of the NO2- pool was similar to
that of the NO pool, and the NH; pool became significantly enriched.
At pH 6.5 DNRA was probably also occurring, because the NH4+ pool was
significantly enriched, but NO2- did not accumulate. Nitrification occ
urred in all treatments and contributed 23% of the N2O flux in the tre
atments with the lowest contents of moisture and C. The flux due to ni
trification was low (< 0.1 mu mol N g(-1) h(-1)) under these condition
s and was not detectable in other treatments because of the much highe
r fluxes from NO3- reduction. Many agronomic practices, such as liming
, urea fertilisation and organic manure addition, result in soil pH va
lues > 6.5 at microsites So DNRA as a process for N2O production may b
e much more important that presently realised. The relative contributi
ons of denitrification and DNRA to N2O production are impossible to qu
antify using only N-15-labelled NO3-. Confirmation of simultaneous fer
mentation and denitrification would require bacterial identification a
nd enumeration. (C) 1998 Elsevier Science Ltd. All rights reserved.