DEVELOPMENT AND VALIDATION OF AMOXICILLIN DETERMINATION BY MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY

A micellar electrokinetic capillary chromatography (MECC) method has b een developed and validated for the analysis of amoxicillin and its po tential impurities. The influence of various parameters on the separat ion such as pH and concentration of the buffer, the concentration of s odium dodecyl sulfate (SDS), and organic solvent were investigated. fi nal electrophoresis conditions were as follows: running buffer, 70 mM sodium dihydrogenphosphate, 125 mM SDS and 5% acetonitrile, adjusted t o pH 6.0; length of fused-silica capillary, 44 cm (effective length 36 cm)X50 mu m I.D.; voltage, 15 kV; temperature, 25 degrees C; detectio n wavelength, 230 nm. The method allows amoxicillin to be completely s eparated from its potential impurities. The robustness of the method h as been examined by means of a full-fraction factorial design. The par ameters for validation, namely relative standard deviation, linearity, precision, limit of detection and limit of quantitation are also repo rted. The performance of MECC was compared with that of liquid chromat ography. (C) 1998 Elsevier Science B.V. All rights reserved.