LIQUID-CHROMATOGRAPHY OF JOSAMYCIN PROPIONATE ON POLY(STYRENE-DIVINYLBENZENE)

An isocratic liquid chromatographic method is described using as stati onary phase the very stable poly(styrene-divinylbenzene) PLRP-S (8 mu m, 1000 Angstrom). The mobile phase was acetonitrile-0.2 M phosphate b uffer, pH 10.0-water (52:20:up to 100, v/v/v), delivered at a flow-rat e of 1.0 ml/min. UV detection was performed at 232 nm. The column was heated at 60 degrees C. Josamycin propionate was separated from all im purities which were present in commercial samples. The main impurities were josamycin, leucomycin A4 propionate, josamycin 2',9-dipropionate , josamycin 3 '',9-dipropionate and platenomycin Al propionate. Full f actorial design was applied to evaluate the robustness of the method. (C) 1998 Elsevier Science B.V. All rights reserved.