DETERMINATION OF NORFLOXACIN IN HUMAN PLASMA AND URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND FLUORESCENCE DETECTION

A method for the determination of norfloxacin in human plasma and urin e is described. Plasma samples were deproteinized using acetonitrile. The supernatant was analysed by C-18 HPLC. Fluorescence detection at a n excitation wavelength of 300 nm and an emission wavelength of 450 nm was utilized. The assay was validated in the concentration range of 3 1 to 2507 ng/ml when 0.5-ml aliquots of plasma were handled. The intra -day precision of the spiked quality control samples ranged from +/-0. 37 to +/-4.14% in plasma (concentration range: 70.3-2109.2 ng/ml) and from +/-0.51 to +/-1.56% in urine (concentration range: 7.5-299.4 mu g /ml). The intra-day accuracy obtained for norfloxacin in the quality c ontrol samples ranged from -5.18% to -9.47% in plasma and from -10.56% to -5.91% in urine. The assay has been used to support human pharmaco kinetic studies. (C) 1998 Published by Elsevier Science B.V. All right s reserved.