METABOLIC ENGINEERING - METHODOLOGIES AND FUTURE-PROSPECTS

Attempts to improve the productivity of cellular systems or to increas e metabolite yield often require radical alteration of the flux throug h primary metabolic pathways. However, achieving the desired result of ten proves difficult because the control architectures at key branch p oints have evolved to resist flux changes. Identification and characte rization of these metabolic nodes is a prerequisite to rational metabo lic engineering.