BIOSYNTHESIS OF GLUCOAMYLASE FROM ASPERGILLUS-NIGER BY SOLID-STATE FERMENTATION USING TEA WASTE AS THE BASIS OF A SOLID SUBSTRATE

Ten waste, supplemented with minerals, was used as the solid substrate for the synthesis of glucoamylase by a strain of Aspergillus niger NC IM 1248. Maximum glucoamylase synthesis [198.4 IU/g dry substrate (gds )] was obtained on the mineralised tea waste at 96 h with an inoculum, initial moisture and pH of 4%, 60% and 4.5, respectively at 30 degree s C. Among different carbon sources sucrose favoured the increase of g lucoamylase synthesis (212.6 IU/gds) in 96 h, whereas among different nitrogen soul ces, malt extract gave the highest enzyme titre (226.0 I U/gds) at 72 h. (C) 1998 Elsevier Science Ltd. All rights reserved.