Aerobically activated sludge from a pig slaughterhouse wastewater-trea
tment plant was mixed with 0.35 or 0.5% (wt/wt) dextrose, with 0.5% (w
t/wt) dextrose and 0.05 or 0.2% (v/v) formic acid, or with 2.5% (wt/wt
) molasses and 0.1% (v/v) formic acid, and was pasteurized at 95 degre
es C for 5 min. After cooling to approximately 20 degrees C the sludge
was inoculated with 10(6)-10(7) cfu Lactobacillus plantarum/g sludge.
Fermentation was performed at 20 degrees C for 21 days. When 0.05% (v
/v) formic acid or less was added the initial pH of the sludge was fav
ourable for the germination and outgrowth of clostridia spores and res
ulted in amino acid breakdown. In the case of the addition of a 2% (v/
v) formic acid and 0.5% (wt/wt) dextrose the initial pH was below 4 an
d the number of lactobacilli did not substantially increase. No amino
acid breakdown was observed. Concerning the safety of the fermented pr
oduct, it was concluded that pasteurized activated sludge from a pig s
laughterhouse can be effectively fermented into a stable product, suit
able for animal feed purposes, with 2.5% (wt/wt) molasses, 0.1% (v/v)
formic acid and L. plantarum as the inoculum. (C) 1998 Elsevier Scienc
e Ltd. All rights reserved.