A COMPARISION OF 2 METHODS TO RECOVER PHAGES FROM SOIL SAMPLES

Environmental contamination caused by viruses has received extensive i nterest. The adsorption of viruses in soil can influence the extent of groundwater pollution. Many methods have been applied to detecting vi ruses in soil samples. Different elution methods lean to differences o f viral titers. In this study, two elution methods were compared glyci ne buffer and beef extract of phages from a soil sample. Experimental results indicated that, for both methods, the phage recovery increased with art increasing contact time between phages and soil sample. The phage recovery for the glycine buffer method increased only slightly w hen the elution time was increased from 2 to 10 min. However the phage recovery for the beef extract method was higher when the elution time was increased to 6 min. At an elution time of 2 min, the glycine buff er method yielded a higher phage recovery than the beef extract method . Although both elution methods closely resembled each other in terms of the phage titers of an environmental sample, the glycine buffer met hod was simple, faster and would be more appropriate for detecting and enumerating phage when many soil or sediment samples are employed. (C ) 1998 Elsevier Science Ltd. All rights reserved.