A METHOD TO SCORE MICRONUCLEI IN-VIVO USING CYTOCHALASIN B-INDUCED CYTOKINESIS BLOCK

Authors
DEVI PU RAO BSS KAMATH R
Citation
Pu. Devi et al., A METHOD TO SCORE MICRONUCLEI IN-VIVO USING CYTOCHALASIN B-INDUCED CYTOKINESIS BLOCK, Mutation research. Fundamental and molecular mechanisms of mutagenesis, 401(1-2), 1998, pp. 33-37
Citations number
10
Categorie Soggetti
Genetics & Heredity",Toxicology,"Biothechnology & Applied Migrobiology
Journal title
Mutation research. Fundamental and molecular mechanisms of mutagenesis
ISSN journal
13861964 → ACNP
Volume
401
Issue
1-2
Year of publication
1998
Pages
33 - 37
Database
ISI
SICI code
1386-1964(1998)401:1-2<33:AMTSMI>2.0.ZU;2-O
Abstract
The present paper describes an in vivo micronucleus assay using Cytoch alasin B (CyB). Mice bearing three different rumours, fibrosarcoma (Sw iss albino mice), B16 F1 melanoma(C57 BL) and Ehrlich ascites carcinom a (Swiss albino mice), were injected with repeated doses of CyB at dif ferent time intervals and binucleate cells were scored at 24, 36, 48, 60 and 72 h after CyB injection. Tt was found that three doses of 3 2 + 2 mg/kg CyB administered intraperitoneally (i.p.) at 12-h interval s effectively blocked cytokinesis. The maximum number of binucleated c ells (BNC) was scored at 60 h after the last CyB dose. This dose sched ule was also effective in scoring micronuclei in BNC after irradiation . (C) 1998 Elsevier Science B.V. All rights reserved.