VOLTAMMETRIC DETERMINATION OF SEROTONIN IN HUMAN BLOOD USING A CHEMICALLY-MODIFIED ELECTRODE

A Nafion(R)/ruthenium-oxide pyrochlore chemically modified electrode ( CME) was used for the determination of serotonin in human blood by squ are-wave voltammetry. Compared to a bare glassy carbon electrode, the chemically modified electrode exhibits a marked enhancement of the cur rent response. With 45 s preconcentration time at a potential of -0.3 V vs. Ag/AgCl, linear calibration curves are obtained in pH 9 ammonia buffer over 0.01-0.5 mu M and 2-20 mu M with a detection limit (3 sigm a) of 2 nm. The chemically modified electrode was applied for the meas urement of serotonin in human blood. (C) 1998 Elsevier Science B.V. Al l rights reserved.