AMPEROMETRIC BIOSENSOR FOR URIC-ACID BASED ON URICASE-IMMOBILIZED SILK FIBROIN MEMBRANE

Citation
Yq. Zhang et al., AMPEROMETRIC BIOSENSOR FOR URIC-ACID BASED ON URICASE-IMMOBILIZED SILK FIBROIN MEMBRANE, Analytica chimica acta, 369(1-2), 1998, pp. 123-128
Citations number
13
Categorie Soggetti
Chemistry Analytical
Journal title
ISSN journal
00032670
Volume
369
Issue
1-2
Year of publication
1998
Pages
123 - 128
Database
ISI
SICI code
0003-2670(1998)369:1-2<123:ABFUBO>2.0.ZU;2-F
Abstract
An amperometric urate sensor based on a uricase-immobilized silk fibro in membrane and an oxygen electrode in flow injection analysis are des cribed in the present paper. The biosensor shows that recoveries of ur ic acid in human serum and urine are in the range of 94.2-102.6% and 9 2.5-97.9%, respectively. The relative standard deviations (RSDs) for r epeatedly monitoring standard urate solution, human serum and urine ar e 2.37, 3.72 and 2.95%, respectively, based on 100 measurements. The u rate sensor based on the uricase-immobilized membrane is capable of de tecting 60-70 human serum samples per hour. Moreover, a piece of urica se-immobilized fibroin membrane used at the sensor could not only be s tored for over 2 years, but also repeatedly monitored more than 1000 t imes for biosamples such as human serum or urine. (C) 1998 Elsevier Sc ience B.V. All rights reserved.