REYNOSIN FROM SASSUREA-LAPPA AS INHIBITOR ON CINC-1 INDUCTION IN LPS-STIMULATED NRK-52E CELLS

An inhibitor on CINC-1 (cytokine-induced neutrophil chemoattractant-1) induction in LPS-stimulated rat kidney epithelioid NRK-52E cells was purified from the roots of Sassurea lappa Clarke, a herbal medicine us ed in Korean traditional prescriptions for gastric intestinal diseases by a variety of column chromatographic procedures. The inhibitor was identified as reynosin, a sesquiterpene lactone isolated and character ized previously from Ambrosia confertiflora DC., and Magnolia grandifl ora L. Reynosin exhibited a dose-dependent inhibition on CINC-1 induct ion in LPS-stimulated NRK-52E cells, where 50 % of inhibitory effect w as shown at the concentration of about 1 mu M.