LEPROSY VACCINE - INFLUENCE OF DISSOLVED-OXYGEN LEVELS ON GROWTH OF ACANDIDATE STRAIN (MYCOBACTERIUM-W), AND STORAGE STABILITY OF THE VACCINE

Citation
A. Mukhopadhyay et al., LEPROSY VACCINE - INFLUENCE OF DISSOLVED-OXYGEN LEVELS ON GROWTH OF ACANDIDATE STRAIN (MYCOBACTERIUM-W), AND STORAGE STABILITY OF THE VACCINE, Vaccine, 16(13), 1998, pp. 1344-1348
Citations number
25
Categorie Soggetti
Veterinary Sciences",Immunology,"Medicine, Research & Experimental
Journal title
ISSN journal
0264410X
Volume
16
Issue
13
Year of publication
1998
Pages
1344 - 1348
Database
ISI
SICI code
0264-410X(1998)16:13<1344:LV-IOD>2.0.ZU;2-V
Abstract
The growth of Mycobacterium w, a candidate strain for leprosy vaccine in submerged culture, was inhibited by the presence of over 40% oxygen saturation in the medium. Intracellular levels of superoxide dismutas e and catalase were very low in the beginning. However; under controll ed oxygenation, these levels increased with time. The augmentations of these antioxidant enzymes were associated with the elevated oxygen co nsumption by the culture. By maintaining the oxygen level below 20% du ring 6-day culture, it was possible to grow Mycobacterium w in five pr oduction batches up to a cell density of 3.7 +/- 0.70 x 10(9) bacilli ml(-1). The shelf life of the vaccine produced in different batches wa s more than 2 years, both at 4 degrees C and at 26 degrees C. This pro vides a cost effective, unit culture technology for the production of this candidate leprosy vaccine from a nonpathogenic organism, which wi ll facilitate use winespread use of the vaccine. (C) 1998 Elsevier Sci ence Ltd. All rights reserved.