A. Mukhopadhyay et al., LEPROSY VACCINE - INFLUENCE OF DISSOLVED-OXYGEN LEVELS ON GROWTH OF ACANDIDATE STRAIN (MYCOBACTERIUM-W), AND STORAGE STABILITY OF THE VACCINE, Vaccine, 16(13), 1998, pp. 1344-1348
Citations number
25
Categorie Soggetti
Veterinary Sciences",Immunology,"Medicine, Research & Experimental
The growth of Mycobacterium w, a candidate strain for leprosy vaccine
in submerged culture, was inhibited by the presence of over 40% oxygen
saturation in the medium. Intracellular levels of superoxide dismutas
e and catalase were very low in the beginning. However; under controll
ed oxygenation, these levels increased with time. The augmentations of
these antioxidant enzymes were associated with the elevated oxygen co
nsumption by the culture. By maintaining the oxygen level below 20% du
ring 6-day culture, it was possible to grow Mycobacterium w in five pr
oduction batches up to a cell density of 3.7 +/- 0.70 x 10(9) bacilli
ml(-1). The shelf life of the vaccine produced in different batches wa
s more than 2 years, both at 4 degrees C and at 26 degrees C. This pro
vides a cost effective, unit culture technology for the production of
this candidate leprosy vaccine from a nonpathogenic organism, which wi
ll facilitate use winespread use of the vaccine. (C) 1998 Elsevier Sci
ence Ltd. All rights reserved.