MAKING GENES GREEN - CREATING GREEN FLUORESCENT PROTEIN (GFP) FUSIONSWITH BLUNT-END PCR PRODUCTS

Authors
LO W RODGERS W HUGHES T
Citation
W. Lo et al., MAKING GENES GREEN - CREATING GREEN FLUORESCENT PROTEIN (GFP) FUSIONSWITH BLUNT-END PCR PRODUCTS, BioTechniques, 25(1), 1998, pp. 94
Citations number
11
Categorie Soggetti
Biology,"Biochemical Research Methods
Journal title
BioTechniques → ACNP
ISSN journal
07366205
Volume
25
Issue
1
Year of publication
1998
Database
ISI
SICI code
0736-6205(1998)25:1<94:MGG-CG>2.0.ZU;2-W
Abstract
The jellyfish green fluorescent protein (GFP) has proven to be a usefu l tool in protein localization and trafficking studies. Fused to GFP, a protein of interest can be visualized and tracked in vivo through fl uorescence microscopy. However; the process of making these fusion pro teins is often tedious and painstaking. Here, we describe a simple and quick method for creating GFP fusion proteins using blunt-end PCR pro duct ligation.