S. Ishino et al., LOSS OF MATERIAL FROM CHROMOSOME ARM 1P DURING MALIGNANT PROGRESSION OF MENINGIOMA REVEALED BY FLUORESCENT IN-SITU HYBRIDIZATION, Cancer, 83(2), 1998, pp. 360-366
BACKGROUND. Atypical and anaplastic meningiomas tend to recur and to i
nvade adjacent brain, bone, and skin. They also can metastasie to extr
acranial organs such as the lung, liver, or bone, causing death. Recen
t reports have indicated that allelic deletion of chromosome 1p is ass
ociated with malignant progression of meningiomas. METHODS. Cytogeneti
c analysis of 37 meningiomas was performed using double-target fluores
cent in situ hybridization (FISH) and focusing on chromosome arm 1p. T
he meningioma series included 17 benign meningiomas, 11 atypical menin
giomas, and 9 anaplastic meningiomas. FISH was performed with pericent
romeric (1q12) and subtelomeric (1p36) DNA probes to cell nuclei prepa
red from surgically extirpated tumor samples. RESULTS. A high incidenc
e of deletion of at least part of 1p was observed in 60.0% of atypical
and 85.7% of anaplastic meningiomas. Furthermore, statistically signi
ficant differences were found with respect to these data between benig
n versus atypical/anaplastic meningiomas. In four cases both primary a
nd recurrent tumors from the same patient also were investigated for a
llelic status. CONCLUSIONS. The results of the current study support t
he existence of tumor suppressor gene(s) on 1p associated with maligna
nt progression of meningioma, and suggest that detection of the alleli
c status of chromosome 1p by FISH may assist physicians in predicting
the clinical prognosis of patients affected by this type of brain tumo
r. (C) 1998 American Cancer Society.