Cottage cheese and yogurt product quality and process control would im
prove if plant operators had a stable and sanitary device for pH measu
rement. The objectives of this research were to determine the effects
of pH, protein, fat, and added calcium on diffuse reflectance of milk
in the wavelength range of 350 to 990 nm and determine if milk pH corr
elates with diffuse reflectance over the range of treatments tested. D
iffuse reflectance of milk was measured at pH levels in the range of 5
.5 to 7.0 and at varying levels of fat, protein, and added calcium con
centrations. Diffuse reflectance was found to decrease with pH, increa
se linearly with protein and fat concentration, be affected by milk pr
etreatment, and be affected by the interaction between calcium and pro
tein. A pH prediction model which included reflectance data and produc
t variables (fat, protein, pre-heat treatment) explained 74.4% of the
variance and had a standard error of 0.29 pH units. Because milk const
ituents and pretreatment affect diffuse reflectance, this method would
be limited in applicability to systems where factors such as fat, pro
tein, calcium, and pretreatment would be relatively constant, such as
in yogurt and cottage cheese production.