ISOLATION AND CHARACTERIZATION OF THE LECTIN FROM THE GREEN MARINE ALGA ULVA-LACTUCA L

Citation
Ah. Sampaio et al., ISOLATION AND CHARACTERIZATION OF THE LECTIN FROM THE GREEN MARINE ALGA ULVA-LACTUCA L, Botanica marina, 41(4), 1998, pp. 427-433
Citations number
36
Categorie Soggetti
Marine & Freshwater Biology","Plant Sciences
Journal title
ISSN journal
00068055
Volume
41
Issue
4
Year of publication
1998
Pages
427 - 433
Database
ISI
SICI code
0006-8055(1998)41:4<427:IACOTL>2.0.ZU;2-H
Abstract
A lectin from the green marine alga Ulva lactuca was isolated by a com bination of affinity chromatography on porcine stomach mucin-Sepharose 6B and gel filtration on Sephadex G-50. Ulva lactuca lectin agglutina ted various animal and human erythrocytes in the following order O>A(1 )>B. The haemagglutinating activity was inhibited by L-fucose, fucoida n, porcine stomach mucin and bovine submaxillary gland mucin. The lect in was stable at variations of pH and temperature and divalent cation dependent. The M-r of U. lactuca agglutinin, determined by gel filtrat ion was 8370, while SDS-PAGE gave one single protein band with a M-r o f 17120. The lectin was shown to be rich in acidic and hydroxyl amino acids, but low in basic amino acids.