Purpose: To evaluate the microleakage of a universal hydrophilic adhes
ive (OptiBond dual-cure) by itself as a pit and fissure sealant compar
ed to that of an ionomeric product (VariGlass) and two hydrophobic sea
lants (Delton and Fluroshield). Materials and Methods: Sixty-four extr
acted human third molars were sealed with one of these four materials
under either a conventional procedure (free of humidity) or after cont
amination of the etched surfaces with 3 mu l of human plasma. The seal
ed teeth were subjected to 1,500 thermocycles (1 min/5-55 degrees C) a
nd to 450,000 mechanical cycles under an 8.5 kgf average load at 37 de
grees C. The tooth crowns were partially coated with nail varnish, imm
ersed in a 50% aqueous solution of AgNO3 for 2 h in darkness, washed a
nd then developed for 8 h under fluorescent light. The teeth were sect
ioned and dye penetration was scored according to a 4-point scale by f
our trained professionals. Results: An ANOVA suggested that under no h
umidity conditions, the average score for VariGlass (1.92 +/- 0.36) wa
s significantly higher (P=0.0006) than that corresponding to the three
remaining materials (1.28 +/- 0.20) which were considered equivalent.
Under humid (plasma contaminated) conditions, there was complete loss
of Delton and Fluroshield in all cases, whereas the average score for
VariGlass (1.98 +/- 0.36) was slightly higher (P=0.0444) than that fo
r OptiBond (1.62 +/- 0.36). These results suggest that OptiBond may be
used by itself as a pit and fissure sealant instead of the combinatio
n of adhesive plus sealant.