CAPILLARY ELECTROPHORETIC ANALYSIS OF GINSENG POLYPEPTIDE

A ginseng polypeptide (GPP) found in ginseng roots and its modified pe ptides were tested by capillary zone electrophoresis (CZE) under acidi c as well as basic conditions. Modified peptides were synthesized for three purposes: (i) to analyze their functions in the first three acid ic amino acid residues, (ii) to analyze their functions in three seque nced glycines, and (iii) to analyze the length of side chains in acidi c amino acids. The roles of glycines, acidic amino acids and amino aci d side chains in the binding of Mg2+ were studied at pH values less th an 7.0. The migration times of GPP varied with the pH of various elect rophoresis buffers, and electrophoresis patterns were significantly ch anged between pH 7.0-7.5. Based on the electrophoretic analysis, it wa s concluded that the binding mechanisms for Mg2+ or conformations of G PP changed between low pH and high pH.