CONTINUOUS-FLOW ISOELECTRIC-FOCUSING FOR PURIFICATION OF PROTEINS

To estimate the feasibility of isoelectric focusing in a continuous mo de, the conditions for obtaining stable and linear pH gradients were f irst investigated, using a mixture of carrier ampholytes. Then the ope rating parameters allowing the isoelectric focusing of bovine serum al bumin as model protein were determined. A theoretical approach was use d to find conditions minimizing the electrohydrodynamic effect, mainly responsible for protein dispersion, for the separation of bovine seru m albumin and cr-lactalbumin from bovine milk.