SUBTYPING OF TRANSFERRIN BY ISOELECTRIC-FOCUSING IN IMMOBILIZED PH GRADIENTS

Isoelectric focusing in immobilized pH gradients ranging from pH 5.05 to 5.60 was used to study the distribution of transferrin (Tf) subtype s and their gene frequencies from 188 unrelated healthy donors of the Han population in Beijing. Six phenotypes (TfC1, TfC2, TfC1C2, TfC1C3, TfC1D(chi) and TfC2D(chi)) were detected and TfC3 has never before b een reported in the Han population. Gene frequencies were as follows: TfC1 = 0.7420, Tf*C2 = 0.2420, Tf*C3 =0.0027, Tf*Dchi = 0.0133. There is good agreement between the observed and expected values correspond ing to the Hardy-Weinberg equilibrium (Sigma chi(2) = 0.9183, df = 2, 0.5 < P < 0.75). The allele frequencies for TfC1, Tf*C2 and Tf*Dchi a greed with those previously reported for the Chinese Han population.