INCORPORATION OF DISPERSION INTO THE THEORY OF ELECTROPHORESIS OF DNAAND ITS COMPLEXES

Incorporation of the dispersion coefficient into the theory of the mob ility-shift assay for DNA-protein complexes was highly successful larg ely due to increased mathematical rigor. A model simulating electropho retic migration of DNA across the phase boundary between the initial z one of macromolecule and the gel lane predicts the peak asymmetry obse rved experimentally. It also predicts that, under the agency of the di spersion coefficient, the peak will become progressively more symmetri cal during migration along the gel lane.