AMPLIFICATION OF MICROSATELLITES ADAPTED FROM HUMAN SYSTEMS IN FECAL DNA OF WILD HANUMAN LANGURS (PRESBYTIS-ENTELLUS)

Microsatellite systems originally established for human DNA were utili zed for paternity testing from faecal DNA in a natural population of H anuman langurs (Presbytis entellus). Thirty-two primer pairs were appl ied to amplify DNA obtained from langur faeces. Twenty-two of these pr imer pairs yielded specific amplification products and 11 loci were po lymorphic. Allele distributions and heterozygosity rates were determin ed for five systems. Genetic information from these five systems was s ufficient for paternity exclusion in 46 out of 52 cases. Results were consistent enough to allow genotyping from faeces, although sometimes only one allele was amplified in a heterozygous individual. In conclus ion, relationship analyses from faeces are possible in spite of the ev olutionary distance between humans and langurs.