THE COMBINATION OF INDIAN INK STAINING WITH IMMUNOCHEMILUMINESCENCE DETECTION ALLOWS PRECISE IDENTIFICATION OF ANTIGENS ON BLOTS - APPLICATION TO THE STUDY OF GLYCOSYLATED BARLEY STORAGE PROTEINS

The localization after blotting of specific spots in two-dimensional e lectrophoretic protein pattern was achieved using, in that order, Indi an ink protein staining and immunodetection with chemiluminescence on the same membrane. Indian ink did not inhibit significantly the antibo dy reactions even after overnight staining. It produces permanent stai ning that did not quench the chemiluminescent signal, recorded on a fi lm. This allowed perfect matching between the specific and the total p rotein patterns. The procedure was applied to the identification of gl ycoproteins present in barley storage protein preparations.