2-DIMENSIONAL ELECTROPHORESIS OF PROTEINS IN AN IMMOBILIZED PH 4-12 GRADIENT

For checking theoretical two-dimensional (2-D) maps derived from seque nced genomes, indicating that nonnegligible amounts of proteins up to pH 12 are to be expected, a wide-range immobilized pH 4-12 gradient wa s generated. Depending on the extraction method of sample preparation, proteins with pls up to pH 12 are detected in a single gel. Highly re producible protein patterns focused to the steady state with round-sha ped spots up to pH 12 are obtained with the standard protocol original ly described in 1988 (Gorg et al., Electrophoresis 1988, 9, 531-546).