CROSS-REACTIVITY OF AN ANTI-OKADAIC ACID ANTIBODY TO DINOPHYSISTOXIN-4 (DTX-4), DINOPHYSISTOXIN-5 (DTX-5), AND AN OKADAIC ACID DIOL ESTER

The cross-reactivity of the 6/50 monoclonal anti-okadaic acid antibody (mAb) to the recently discovered diarrhetic shellfish poisoning (DSP) metabolites dinophysistoxin-4 (DTX-4), dinophysistoxin-5 (DTX-5), and an okadaic acid (OA) diol ester was determined using a competitive in direct enzyme-linked immunosorbent assay (ELISA). The reactivity of th e antibody to these molecules was compared to that with OA; the 6/50 m Ab recognized all of these DSP compounds with equal sensitivity within the working range of the antibody (10-100 nM for GA). This confirms t he ability of the antibody to detect all DSP compounds when used in an alyses including ELISA and immunocytochemistry. Crown copyright (C) 19 98 Published by Elsevier Science Ltd. All rights reserved.