N. Yamamoto et al., CLASSIFICATION OF LACTOBACILLUS-HELVETICUS STRAINS BY IMMUNOLOGICAL DIFFERENCES IN EXTRACELLULAR PROTEINASES, Bioscience, biotechnology, and biochemistry, 62(6), 1998, pp. 1228-1230
Polyclonal antibodies against an extracellular proteinase of Lactobaci
llus helveticus CP53 were raised. The antibodies reacted with a 170-kD
a enzyme with activity and a 53-kDa protein that seemed to be a degrad
ation product of the 170-kDa proteinase from results of immunoblotting
;. The antibodies reacted also with a 45-kDa extracellular proteinase
of L. helveticus CP790. However, monoclonal antibodies to the CP790 pr
oteinase did not react with the proteinase of L, helveticus CP53. Seve
nteen strains of L. helveticus were tested for immunological reactivit
y with the two kinds of antibodies. The strains all had the same react
ivity as either strain CP53 or strain CP790. Eleven strains with the 4
5-kDa proteinase were identified as L. helveticus biovar jugurti becau
se they did not ferment maltose, four other strains with the 170- and
53-kDa proteins were identified as L. helveticus biovar helveticus bec
ause they fermented maltose, The remaining two strains dit not fit thi
s pattern; they had both the 170- and 53-kDa proteins, but classificat
ion by their sugar utilization showed them to be L. helveticus biovar
jugurti.