MALDI-TOF ANALYSIS OF MIXTURES OF 3-DEOXYANTHOCYANIDINS AND ANTHOCYANINS

Authors
SUGUI JA BONHAM C LO SC WOOD KV NICHOLSON RL
Citation
Ja. Sugui et al., MALDI-TOF ANALYSIS OF MIXTURES OF 3-DEOXYANTHOCYANIDINS AND ANTHOCYANINS, Phytochemistry, 48(6), 1998, pp. 1063-1066
Citations number
21
Categorie Soggetti
Biology,"Plant Sciences
Journal title
Phytochemistry → ACNP
ISSN journal
00319422
Volume
48
Issue
6
Year of publication
1998
Pages
1063 - 1066
Database
ISI
SICI code
0031-9422(1998)48:6<1063:MAOMO3>2.0.ZU;2-S
Abstract
The mass spectrometric analysis of 3-deoxyanthocyanidins and anthocyan ins present in crude extracts from sorghum plant tissue was carried ou t by matrix-assisted laser desorption ionization mass spectrometry (MA LDI). Sensitivities to as low as 5 pmol/mu l were easily attained for pure samples of the anthocyanidin, pelargonidin, and the anthocyanin, malvin. Sensitivities to the level of 15 pmol/mu l were attained for 3 -deoxyanthocyanidins present in crude extracts from sorghum plant tiss ue. Through its capability for analyzing very small quantities of thes e compounds in unpurified samples, MALDI.provided a sensitive means fo r the detection of these flavonoid pigments in plant tissues. (C) 1998 Elsevier Science Ltd. All rights reserved.