EFFECT OF EXTENDER SOLUTIONS AND DILUTION ON MOTILITY AND FERTILIZINGABILITY OF EASTERN OYSTER SPERM

Citation
Cg. Paniaguachavez et al., EFFECT OF EXTENDER SOLUTIONS AND DILUTION ON MOTILITY AND FERTILIZINGABILITY OF EASTERN OYSTER SPERM, Journal of shellfish research, 17(1), 1998, pp. 231-237
Citations number
29
Categorie Soggetti
Fisheries,"Marine & Freshwater Biology
ISSN journal
07308000
Volume
17
Issue
1
Year of publication
1998
Pages
231 - 237
Database
ISI
SICI code
0730-8000(1998)17:1<231:EOESAD>2.0.ZU;2-X
Abstract
Optimization of conditions for short-term storage of gametes is import ant for the production of seedstock and genetic management of broodsto ck in aquaculture. We conducted a series of experiments to evaluate re frigerated storage of sperm of the Eastern oyster (Crassostrea viginic a). Our objectives were to: 1) compare motility of oyster sperm suspen ded in artificial seawater (ASW) at five osmotic pressures (22, 203, 4 03, 601, or 833 mOsm/kg) over 24 h; 2) compare motility of sperm suspe nded in solutions of ASW, Hanks' balanced salt solution (HBSS), or DCS B4 solution tall at 833 mOsm/kg) over 4 days of refrigerated storage; 3) compare motility of sperm suspended in ASW or HBSS (833 mOsm/kg) in six ratios of sperm:extender (1:0, 1:1, 1:3, 1.7, 1:15, or 1:31) over 4 days of refrigerated storage, and 4) compare motility and fertilizi ng capacity of oyster suspended in ASW (200 and 830 mOsm/kg), ASW with 6% glycine (ASW+G), HBSS, and calcium-free HBSS (C-F HBSS) tall at 83 0 mOsm/kg). Significant differences (p < 0.001) were found in the moti lity of sperm suspended in ASW of different osmotic pressures. No sign ificant differences (p = 0.267) were found in motility of sperm suspen ded in ASW, HBSS, or DCSB4. Significant differences (p < 0.001) were f ound in the motility of sperm suspended in different ratios of sperm:e xtender. The highest motility was found in undiluted sperm and the low est in the 1:31 dilution for sperm suspended in ASW or HBSS. Significa nt differences (p = 0.0001) were found in motility of sperm suspended in various extenders. The highest motility was found in sperm diluted in CF-HBSS (96%), and the lowest in sperm diluted in ASW at 200 mOsm/k g (12%). The highest percent fertilization (48%) las measured by larva l development at 12 h) was obtained when eggs were fertilized with spe rm diluted in C-F HBSS. These results indicate that for storage (4 day s), it is best to leave sperm samples undiluted. However, when sperm s amples are diluted for use within 24 h, it is best to maintain high sp erm concentrations and to use C-F HBSS as an extender.