AN APPROACH TO THE EVALUATION AND COMPARISON OF REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY STATIONARY PHASES

An approach to the evaluation and comparison of reversed-phase high-pe rformance liquid chromatography stationary phases with particular emph asis on data analysis and presentation is described. Assessment is bas ed on the peak efficiency, asymmetry (USP tailing factor) and relative retention properties shown by 24 basic compounds having a wide range of structural and physico-chemical properties. A novel approach-to dat a normalisation and presentation is described. This overcomes the prob lems associated with the quality of the column packing process, as wel l as differences in stationary phase selectivity which in conjunction with extra column band broadening effects can make comparisons meaning less. (C) 1998 Published by Elsevier Science B.V. All rights reserved.