DETERMINATION OF 2-HYDROXYPHENYLACETIC ACID (2HPAA) IN URINE AFTER ORAL AND PARENTERAL ADMINISTRATION OF COUMARIN BY GAS-LIQUID-CHROMATOGRAPHY WITH FLAME-IONIZATION DETECTION
I. Meineke et al., DETERMINATION OF 2-HYDROXYPHENYLACETIC ACID (2HPAA) IN URINE AFTER ORAL AND PARENTERAL ADMINISTRATION OF COUMARIN BY GAS-LIQUID-CHROMATOGRAPHY WITH FLAME-IONIZATION DETECTION, Journal of pharmaceutical and biomedical analysis, 17(3), 1998, pp. 487-492
The urinary excretion of 2-hydroxyphenylacetic acid (2HPAA) was studie
d in human volunteers after oral and parenteral doses of coumarin. The
presence of 2HPAA in the urine was confirmed by gas chromatography ma
ss spectroscopy (GC MS). Mass spectra of reference material and sample
s are presented. The determination of 2HPAA was carried out by GC with
flame-ionization detection. Prior to analysis samples were extracted
into ethyl ether and the analytes were derivatized with trimethylpheny
lammonium hydroxide. A calibration range from 0.3 to 150 mu g ml(-1) w
as established using 3-hydroxyphenyl acetic acid (3HPAA) as an interna
l standard. On average less than 10% of the coumarin administered were
excreted into the urine in the form of 2HPAA. (C) 1998 Elsevier Scien
ce B.V. Al rights reserved.