DETERMINATION OF 2-HYDROXYPHENYLACETIC ACID (2HPAA) IN URINE AFTER ORAL AND PARENTERAL ADMINISTRATION OF COUMARIN BY GAS-LIQUID-CHROMATOGRAPHY WITH FLAME-IONIZATION DETECTION

Citation
I. Meineke et al., DETERMINATION OF 2-HYDROXYPHENYLACETIC ACID (2HPAA) IN URINE AFTER ORAL AND PARENTERAL ADMINISTRATION OF COUMARIN BY GAS-LIQUID-CHROMATOGRAPHY WITH FLAME-IONIZATION DETECTION, Journal of pharmaceutical and biomedical analysis, 17(3), 1998, pp. 487-492
Citations number
10
Categorie Soggetti
Pharmacology & Pharmacy","Chemistry Analytical
ISSN journal
07317085
Volume
17
Issue
3
Year of publication
1998
Pages
487 - 492
Database
ISI
SICI code
0731-7085(1998)17:3<487:DO2A(I>2.0.ZU;2-5
Abstract
The urinary excretion of 2-hydroxyphenylacetic acid (2HPAA) was studie d in human volunteers after oral and parenteral doses of coumarin. The presence of 2HPAA in the urine was confirmed by gas chromatography ma ss spectroscopy (GC MS). Mass spectra of reference material and sample s are presented. The determination of 2HPAA was carried out by GC with flame-ionization detection. Prior to analysis samples were extracted into ethyl ether and the analytes were derivatized with trimethylpheny lammonium hydroxide. A calibration range from 0.3 to 150 mu g ml(-1) w as established using 3-hydroxyphenyl acetic acid (3HPAA) as an interna l standard. On average less than 10% of the coumarin administered were excreted into the urine in the form of 2HPAA. (C) 1998 Elsevier Scien ce B.V. Al rights reserved.