SYNTHESIS OF GAMMA-PHOSPHATE-LINKED NUCLEOSIDE AFFINITY-CHROMATOGRAPHY RESINS FOR PROTEIN-PURIFICATION, INCLUDING RIBONUCLEOSIDE TRIPHOSPHATE REDUCTASE

Authors
SUTO RK WHALEN MA BENDER BR FINKE RG
Citation
Rk. Suto et al., SYNTHESIS OF GAMMA-PHOSPHATE-LINKED NUCLEOSIDE AFFINITY-CHROMATOGRAPHY RESINS FOR PROTEIN-PURIFICATION, INCLUDING RIBONUCLEOSIDE TRIPHOSPHATE REDUCTASE, Nucleosides & nucleotides, 17(8), 1998, pp. 1453-1471
Citations number
61
Categorie Soggetti
Biology
Journal title
Nucleosides & nucleotides → ACNP
ISSN journal
07328311
Volume
17
Issue
8
Year of publication
1998
Pages
1453 - 1471
Database
ISI
SICI code
0732-8311(1998)17:8<1453:SOGNA>2.0.ZU;2-K
Abstract
Seven nucleotides linked through the gamma-phosphate to diamine hydroc arbons were synthesized and coupled to Sepharose for use in protein pu rification affinity chromatography. The synthesis involved converting the nucleotides to nucleoside-5'-trimetaphosphates using dicyclohexyl carbodiimide, followed by nucleophilic ring opening of the trimetaphos phate with an alpha omega-diamino hydrocarbon to generate a gamma-phos phoamide linkage in each nucleotide.