DEVELOPMENT OF A CHEMILUMINESCENCE METHOD FOR THE SIMULTANEOUS DETERMINATION OF ASCORBIC AND TARTARIC-ACIDS BASED UPON THEIR REACTION WITH CERIUM(IV) IN THE PRESENCE OF RUTHENIUMTRISDIPYRIDINE

A time-resolved chemiluminescent method for the determination of ascor bic and tartaric acid has been developed. The method is based on the d ifferential rate of the chemiluminescent reaction of Ru(bipy)(3)(2+) a scorbic and tartaric acid with Ce(IV). The ascorbic acid system gives the highest chemiluminescence intensity at 2s, whereas the tartaric ac id system gives its most intense chemiluminescence emission at 40s. As corbic acid (1.7x10(-6) similar to 5.2x10(-8) M) and tartaric acid (1. 4x10(-4) similar to 1.4x10(-6) M) in wines were determined simultaneou sly with relative standard deviations of better than 5%(n=5). The dete ction limit of ascorbic acid and art acid were 4.8x10(-8) M and 8.4x10 (-7) M, respectively.