A. Schippers et al., EVALUATION OF THE EFFICIENCY OF MEASURES FOR SULPHIDIC MINE WASTE MITIGATION, Applied microbiology and biotechnology, 49(6), 1998, pp. 698-701
To control the environmentally detrimental impact of acid rock drainag
e, two different countermeasures, layers of acid-buffering materials a
nd sodium dodecyl sulphate addition, were tested for their efficiency
in laboratory percolation experiments. In the experiment with a layer
of calcium bentonite, only the iron output was reduced. The experiment
s with layers of concrete grains demonstrated a decrease of the microb
ial activity as well as a precipitation of heavy-metal ions, whereas t
he cell numbers did not decrease. Furthermore, finely grained concrete
(1-5 mm) formed a water-tight hard-pan (self-sealing layer). In the e
xperiment with 1 mM sodium dodecyl sulphate, all the microorganisms we
re killed and hence metal sulphide dissolution was stopped. With 0.1 m
M sodium dodecyl sulphate only a short, transient inhibition of leachi
ng was achieved. The bacteria remained alive.