PRODUCTION OF UNINFECTIOUS HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 CONTAINING VIRAL PROTEIN-R FUSED TO A SINGLE-CHAIN ANTIBODY AGAINST VIRAL INTEGRASE

Authors
OKUI N KOBAYASHI N KITAMURA Y
Citation
N. Okui et al., PRODUCTION OF UNINFECTIOUS HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 CONTAINING VIRAL PROTEIN-R FUSED TO A SINGLE-CHAIN ANTIBODY AGAINST VIRAL INTEGRASE, Journal of virology, 72(8), 1998, pp. 6960-6964
Citations number
34
Categorie Soggetti
Virology
Journal title
Journal of virology → ACNP
ISSN journal
0022538X
Volume
72
Issue
8
Year of publication
1998
Pages
6960 - 6964
Database
ISI
SICI code
0022-538X(1998)72:8<6960:POUHTC>2.0.ZU;2-6
Abstract
A single-chain antibody (scAb) against human immunodeficiency virus ty pe 1 (HIV-1) integrase was expressed as a fusion protein of scAb and H IV-1 viral protein R (Vpr), together with the HIV-1 genome, in human 2 93T cells. The expression aid not affect virion production much but ma rkedly reduced the infectivity of progeny virions. The fusion protein was found to be incorporated into the virions. The incorporation appea rs to account for the reduced infectivity.