IMPROVED PLATING EFFICIENCY OF LILY (LILIUM X FORMOLONGI HORT) PROTOPLASTS BY SILVER THIOSULFATE ADDED TO ENZYME SOLUTION

Citation
T. Godo et al., IMPROVED PLATING EFFICIENCY OF LILY (LILIUM X FORMOLONGI HORT) PROTOPLASTS BY SILVER THIOSULFATE ADDED TO ENZYME SOLUTION, Ikushugaku Zasshi, 48(2), 1998, pp. 159-161
Citations number
10
Categorie Soggetti
Agriculture,"Plant Sciences
Journal title
ISSN journal
05363683
Volume
48
Issue
2
Year of publication
1998
Pages
159 - 161
Database
ISI
SICI code
0536-3683(1998)48:2<159:IPEOL(>2.0.ZU;2-F
Abstract
Lily (Lilium x formolongi hort.) protoplasts were isolated from merist ematic nodular cell clumps using enzyme solution containing different concentrations of silver thiosulfate (STS), embedded in 0.1% (W/V) gel lan gum - solidified medium containing half strength mineral salts of Murashige and Skoog (1962) medium, 4.1 mu M 4-amino-3,5,6-trichloro-pi colinic acid (picloram) and 0.5 M glucose, and cultured at 25 degrees C in the dark. The highest plating efficiency (17.7%) was obtained whe n the protoplasts were isolated using the enzyme solution containing 1 0 mu M STS.