T. Hansner et al., AMPLIFICATION OF GENOMIC DNA FRAGMENTS OF SARCOCYSTIS-MURIS (APICOMPLEXA) CYST MEROZOITES ENCODING A THIOL (CYSTEINE) PROTEINASE, Parasitology research, 84(7), 1998, pp. 578-582
Parasites of the phylum Apicomplexa (Sporozoa) cause diseases such as
malaria, toxoplasmosis. or intestinal coccidiosis. Invasive stages pos
sess typical apical organelles such as dense granules that harbor a br
oad range of polypeptides that are believed to take part in the parasi
te-host cell interaction, In previous studies a 26-kDa polypeptide of
dense granules from Sarcocystis muris cyst merozoites (bradyzoites) wa
s characterized as a thiol (cysteine) proteinase. In this paper a meth
od is demonstrated to amplify DNA fragments from genomic DNA of S. mur
is cyst merozoites by polymerase chain reaction. which probably code f
or the 26-kDa antigen.