DNA EXTRACTION FROM ASCARIS-SUUM MUSCLE-TISSUE

Authors
DIMITO C BETSCHART B
Citation
C. Dimito et B. Betschart, DNA EXTRACTION FROM ASCARIS-SUUM MUSCLE-TISSUE, Parasitology research, 84(7), 1998, pp. 596-597
Citations number
3
Categorie Soggetti
Parasitiology
Journal title
Parasitology research → ACNP
ISSN journal
09320113
Volume
84
Issue
7
Year of publication
1998
Pages
596 - 597
Database
ISI
SICI code
0932-0113(1998)84:7<596:DEFAM>2.0.ZU;2-8
Abstract
A new method for the extraction of DNA from Ascaris suum muscle has be en developed. It combines a standard SDS-based extraction with a plant DNA extraction procedure. The use of SDS and proteinase It allows the elimination of proteins, while CTAB and polyclar AT eliminate glycoge n and polyphenols. The DNA thus obtained can easily be digested by end onucleases and amplified by PCR.