CULTIVAR IDENTIFICATION AND RELATIONSHIPS IN PISUM-SATIVUM L. BASED ON RAPD AND ISOENZYMES

Citation
P. Samec et al., CULTIVAR IDENTIFICATION AND RELATIONSHIPS IN PISUM-SATIVUM L. BASED ON RAPD AND ISOENZYMES, Biologia plantarum, 41(1), 1998, pp. 39-48
Citations number
35
Categorie Soggetti
Plant Sciences
Journal title
ISSN journal
00063134
Volume
41
Issue
1
Year of publication
1998
Pages
39 - 48
Database
ISI
SICI code
0006-3134(1998)41:1<39:CIARIP>2.0.ZU;2-7
Abstract
Two approaches were used to detect variability within 23 pea cultivars , Eight random amplified polymorphic DNA (RAPD) primers produced 163 b ands (114 polymorphic), whereas five isoenzyme systems produced 25 ban ds (20 polymorphic), Both methods provided good results in finger-prin ting and identified all cultivars tested. Genetic similarity analysis (UPGMA) gave different results from RAPD and isoenzyme data. This coul d reflect different properties of both marker types. These data were a lso analysed using the bootstrap method, which supported the idea of o ne common progenitor of cultivated peas.