From theoretical models, it is now well known that the pulsation motio
n of atmospheric layers located above the photosphere have not the for
m of a standing wave especially in large amplitude pulsating stars suc
h as RR Lyrae stars. In this paper, we report for the first time a wea
k but clear Van Hoof effect between some metallic lines in the atmosph
ere of the brightest RR Lyrae star of the sky: RR Lyrae. This shows th
at the propagation time of the pulsation wave through the atmosphere i
s now measurable with high resolution spectrographs. This provides a p
ower tool to test nonlinear nonadiabatic pulsating models. We also con
firm, but with a better accuracy, the Van Hoof effect with hydrogen li
nes which an formed higher in the atmosphere. Thus our observations pu
t into evidence that large amplitude nonlinear motions of the atmosphe
ric layers already exist at the photospheric level i.e., in the deep a
tmosphere.