Dr. Bobbitt et al., CHEMILUMINESCENT DETECTION OF AMINES AND AMINO-ACIDS USING IN-SITU GENERATED RU(BPY)(3)(3-ELECTROPHORESIS() FOLLOWING SEPARATION BY CAPILLARY), Talanta, 46(4), 1998, pp. 565-572
A new sensitive chemiluminescent detection method for capillary electr
ophoresis is described. Underivatized amines and amino acids were dete
cted following capillary electrophoresis separation by their chemilumi
nescent reaction with Ru(bpy)(3)(3+) generated in situ at 35 mu m carb
on fibers. Detection limits for triethylamine and proline were 5 and 3
fmol, respectively at a SNR of three. The noise limiting the detectab
ility of separated analytes was determined to exist at the level of th
e dark noise limit of the PMT used for these studies and additional no
ise reduction strategies are expected to improve the quantitative aspe
cts of the method. Theoretical plate numbers for proline were approxim
ately 20000. End column addition of Ru(bpy)(3)(2+) coupled with in sit
u generation of Ru(bpy)(3)(3+), has been shown to be compatible with t
he nanoliter elution volumes characteristic of capillary electrophores
is. (C) 1998 Elsevier Science B.V. All rights reserved.