SALTING-OUT EXTRACTION TECHNIQUE FOR PRETREATMENT IN THE LIQUID-CHROMATOGRAPHIC DETERMINATION OF COPPER(II), ALUMINUM(III), IRON(III) AND MANGANESE(II) IN BIOLOGICAL SAMPLES

A solvent extraction method for the simultaneous determination of Cu, Al, Fe and Mn by reversed phase liquid chromatography is presented. Th e metal chelates with 5-chloro-8-quinolinol are extracted into an acet onitrile phase with tetrabutylammonium perchlorate and ammonium sulfat e as salting-out agents, followed by LC separation and determination u sing an ODS column. The experimental parameters such as the compositio n of mobile phase and concentration of the salting-out agent for phase separation have been investigated. The mobile phase is a mixture of 3 :1 (v/v) acetonilrile-0.02 mol l(-1) sodium acetate solution containin g 5 x 10(-3) mol l(-1) 5-chloro-8-quinolinol. The proposed method has been applied to the trace analysis of Cu, Al, Fe and Al in bovine live r and citrus leaves. (C) 1998 Elsevier Science B.V. All rights reserve d.