EXAMINATION OF THE RELATIONSHIP BETWEEN NET EXTINCTION IN ELISA AND VIRUS NEUTRALIZATION METHODS FOR ESTIMATING THE TITER OF NEUTRALIZING ANTIBODIES AGAINST BOVINE HERPESVIRUS-1

Authors
LAZIC S PEROVIC G ASANIN R DRAZIC L MILANOV D VIDIC B
Citation
S. Lazic et al., EXAMINATION OF THE RELATIONSHIP BETWEEN NET EXTINCTION IN ELISA AND VIRUS NEUTRALIZATION METHODS FOR ESTIMATING THE TITER OF NEUTRALIZING ANTIBODIES AGAINST BOVINE HERPESVIRUS-1, Acta veterinaria (Beograd), 48(2-3), 1998, pp. 99-104
Citations number
17
Categorie Soggetti
Veterinary Sciences
Journal title
Acta veterinaria (Beograd) → ACNP
ISSN journal
05678315
Volume
48
Issue
2-3
Year of publication
1998
Pages
99 - 104
Database
ISI
SICI code
0567-8315(1998)48:2-3<99:EOTRBN>2.0.ZU;2-6
Abstract
Specific antibodies (Ab's) against bovine herpesvirus-1 (BHV-1) were d etected by parallel examination of blood samples obtained from cows an d calves, utilizing the ELISA-test and the method of micro virus neutr alization. An adequate regression relationship between net extinction and the values of neutralizing antibody titer was determined by statis tical analysis of the results obtained. However, values for antibody t iters could not be accurately predicted on the on the basis of net ext inction. It was determined that the accuracy rate was 30% for the majo rity of tested titers However, accuracy approched 80% for particular v alues of neutralizing antibody titer.