OXIDATIVE STRESS AND ADVANCING AGE - RESULTS IN HEALTHY CENTENARIANS

OBJECTIVE: Our study aims at investigating the degree of oxidative str ess in centenarians DESIGN: Indices of oxidative stress (reaction prod ucts of malondialdehyde with thiobarbituric acid (TBARS) and lipid hyd roperoxides (LPO)), and plasma concentrations of antioxidant defenses (plasma vitamin E and C concentrations and reduced/oxidized glutathion e ratio (GSH/GSSG)) were determined. SUBJECTS: Eighty-two subjects vol unteered for the study. They were divided into three groups: (1) adult s (<50 years of age, n = 30); (2) aged subjects (70-99 years, n = 30); (3) centenarians (age greater than or equal to 100 years, n = 22). ME ASUREMENTS: TBARS and LPO, plasma vitamin E and C concentrations, and plasma GSH/GSSG ratio were determined. Insulin action was assessed by euglycemic hyperinsulinemic glucose clamp. MAIN RESULTS: TBARS (0.44 /- 0.07 vs 0.31 +/- .05 nmol malondialdehyde/mL plasma, P = .020) and LPO (0.36 +/- 0.05 vs 0.31 +/- .04 mu mol/L, P = .050) were lower in c entenarians than in aged subjects. In contrast, plasma GSH/GSSG ratio (0.82 +/- 0.09 vs 1.17 +/- .06, P = .010), vitamin C (72.3 +/- 4.6 vs 59.4 +/- 3.8 mu mol/L P = .010), and vitamin E (29.1 +/- 2.2 vs 24.4 /- 2.3 mu mol/L P = .050) concentrations were more elevated in centena rians than in aged subjects. Differences in daily vegetable intake, in fasting plasma glucose and free fatty acid (FFA) concentrations, and in insulin action are significant determinants of degree of oxidative stress. A specific genetic background in centenarians might also provi de a possible explanation. CONCLUSIONS: The degree of oxidative stress is lower in healthy centenarians than in aged subjects.