COMPARISON OF BROTH MICRODILUTION METHOD USING HAEMOPHILUS TEST MEDIUM AND AGAR DILUTION METHOD FOR SUSCEPTIBILITY TESTING OF EIKENELLA-CORRODENS

Susceptibility testing of Eikenella corrodens is usually performed by a Mueller-Hinton sheep blood agar dilution (AD) method. However, this method is impractical for testing only a few strains. We compared AD w ith the broth microdilution method using Haemophilus test medium (HTM) in order to determine the susceptibility of 36 clinical isolates off. corrodens to eight antimicrobial agents. MICs obtained by the HTM met hod yielded 95.5 and 84% agreement (within 2 and 1 log, dilutions, res pectively) with those obtained by AD. The HTM method with incubation i n CO2 for 48 h was highly reproducible and constitutes an easy alterna tive for antimicrobial susceptibility testing off. corrodens.