INHIBITION OF RIBOSOMAL-SUBUNIT ASSOCIATION AND PROTEIN-SYNTHESIS BY OLIGONUCLEOTIDES CORRESPONDING TO DEFINED REGIONS OF 18S RIBOSOMAL-RNAAND 5S RIBOSOMAL-RNA
Aa. Azad et al., INHIBITION OF RIBOSOMAL-SUBUNIT ASSOCIATION AND PROTEIN-SYNTHESIS BY OLIGONUCLEOTIDES CORRESPONDING TO DEFINED REGIONS OF 18S RIBOSOMAL-RNAAND 5S RIBOSOMAL-RNA, Biochemical and biophysical research communications (Print), 248(1), 1998, pp. 51-56
Strong complementarity between a conserved sequence near the 3' end of
18S (16S) rRNA of the small ribosomal subunit and a conserved sequenc
e in the 5S rRNA of the large ribosomal subunit supported the suggesti
on that base-paired interaction between the two RNA molecules could be
responsible for the reversible association of ribosomal subunits duri
ng protein synthesis. If this were true then oligonucleotides correspo
nding to defined regions of the 18S and 5S rRNAs should have profound
effects on the association of ribosomal subunits and protein synthesis
. In this report we show that oligonucleotides, corresponding to a def
ined region of eukaryotic 18S rRNA, when bound to wheat embryo 60S rib
osomal subunits, inhibited association with 40S ribosomal subunits and
also inhibited in vitro protein synthesis. Similarly oligonucleotides
corresponding to a defined region of 5S rRNA when bound to 40S riboso
mal subunits also inhibited the formation of 80S ribosomes and in vitr
o protein synthesis. The minimum sequences responsible for the inhibit
ion of ribosomal subunit association and in. vitro protein synthesis c
orresponded to the 5' strand of the m(2)(6)A m(2)(6)A hairpin structur
e near the 3' end of 18S rRNA and nucleotides 91-100 of 5S rRNA which
are complementary to each other. Sequences at identical positions of E
scherichia coil 16S and 5S rRNAs are also complementary to each other.
(C) 1998 Academic Press.