MOLECULAR-CLONING OF RAT PAX4 - IDENTIFICATION OF 4 ISOFORMS IN RAT INSULINOMA CELLS

Authors
TOKUYAMA Y YAGUI K SAKURAI E HASHIMOTO N SAITO Y KANATSUKA A
Citation
Y. Tokuyama et al., MOLECULAR-CLONING OF RAT PAX4 - IDENTIFICATION OF 4 ISOFORMS IN RAT INSULINOMA CELLS, Biochemical and biophysical research communications (Print), 248(1), 1998, pp. 153-156
Citations number
18
Categorie Soggetti
Biology,Biophysics
Journal title
Biochemical and biophysical research communications (Print) → ACNP
ISSN journal
0006291X
Volume
248
Issue
1
Year of publication
1998
Pages
153 - 156
Database
ISI
SICI code
0006-291X(1998)248:1<153:MORP-I>2.0.ZU;2-1
Abstract
Molecular cloning of rat Pax4 cDNA from a rat insulinoma cell line, RI Nm5F, library by PCR-based cloning strategy revealed four isoforms of the protein. Analysis of tissue distribution using Northern blotting a nd RT-PCR showed specific expression of Pax4 mRNA in pancreatic islets and RIN cells. RT-PCR confirmed that the mRNAs of four isoforms are e xpressed in RIN cells. These Pax4 variants may regulate the transcript ional activity of Pax4 during the development of pancreatic islets. (C ) 1998 Academic Press.