NEURITE OUTGROWTH OF DORSAL-ROOT GANGLIA IS DELAYED AND ARRESTED BY ASPIRIN

In this study, the effect of increasing doses of aspirin on the neurit e outgrowth of Dorsal Root Ganglia (DRG) was investigated. DRG were cu ltured in complete medium (DMEM+10% FCS + 100ng/ml NGF +collagen Type1 in substratum in 96 multiwell plate) in the presence of concentration of 1.25, 2.5, 5 and 10 mM aspirin. The neurite outgrowth of DRG was fo llowed in comparison with controls that lack aspirin. 10 mM aspirin tr eated DRG; showed delayed neurite outgrowth and after 7 days it reache d the same DRG neurite outgrowth control wells after 18 hrs. This grow th has delaye approximately one week and showed no further development and in such stage the cells became apoptos. However at concentrations of 1.25, 2.5, 5 mM of aspirin, outgrowth was observed after 18-24 hrs . Although the rate of growth was lower than control, it was not signi ficant. In the other experiment, when DRG cultured for one week in com plete medium then treated with aspirin, at 10 mM, DRG neurite outgrowt h was stopped,while it was continued in the control. It seem that the aspirin affected DRG; became apoptosis. (C) 1998 Academic Press.